Device

Part:BBa_K2080001:Design

Designed by: Haojian Li   Group: iGEM16_SCUT-China_B   (2016-09-22)


bax-sgRNA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed by analyzing the Bax promoter, the gRNA sequence is complementary to the Bax promoter region, 315 bp upstream the transcription start site, and three repeated protospacers upstream the minimal promoter of Bax-1 promoter. Cloned into pSB1C3 using EcoRI and PstI restriction sites.

Source

Sequence of gRNA scaffold: Form BBa_K2080000

Sequence of gRNA: We designed the sequence of gRNA by analyzing the promoter of Bax

Sequence of U6 promoter[1]

References

[1]In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature. 2015 Apr 9;520(7546):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. http://www.ncbi.nlm.nih.gov/pubmed/25830891